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Chromatographic Separations of Inositol Phosphorus Compounds¹

ABSTRACT

A chromatographic technique for the separation of sodium phytate and its enzymatically produced derivatives is described. From a mixture of sodium phytate and derivatives, nine organic phosphorus compounds were separated in sufficient quantities to permit determination of their inositol/phosphorus ratios. Phosphorus was determined colorimetrically following perchloric acid digestion, and inositol was determined by means of a biological assay. The determinations were replicated sufficiently that the number of phosphorus atoms per molecule of inositol was considered to have been determined to within one atom of the true value in all cases. The identity of the nine separated compounds was tentatively established.

¹ Division of Soil Management and Irrigation, B.P.I.S.A.E., A.R.A., U.S.D.A., and Iowa Agricultural Experiment Station, Ames, Iowa, cooperating. Journal Paper no. J-1048, Project 965, of the Iowa Agricultural Experiment Station. Presented before Section III, Soil Science Society of America, State College, Pa., August 30, 1951.