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Single Strains and a Mixture of Rhizobium trifolii for Inoculating True Clovers¹

ABSTRACT

Twenty strains of Rhizobium trifolii were grown separately and equal portions were compounded into a single mixed humus culture. Twelve species of Trifolium were inoculated with this mixed culture, with a single good strain, and with a poor strain. On Trifolium incarnatum and T. fragiferum, the mixed culture was significantly better than the single good strain culture. On T. subterraneum, T. hirtum, and T. lappaceum, the single good strain was significantly better than the mixed culture. On the seven other species, T. repens, T. repens var. ladino, T. pratense, T. hybridum, T. nigrescens, T. glomeratum, and T. resupinatum, no significant differences were found between the single good strain and the mixed culture. The presence of poor strains in the mixed culture apparently had no harmful effect on its efficiency to inoculate the 12 different species of true clovers.

¹ Contribution from Division of Soil Management and Irrigation, Bureau of Plant Industry, U.S.D.A., Beltsville, Md. Presented before Section III, Soil Science Society of America, State College, Pa., August 29, 1951.

² Senior Bacteriologist and Junior Plant Physiologist, respectively.