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The Effects of Fungicides, Insecticides, Herbicides, and Fertilizer Salts on the Respiration of Root Tips of Tree Seedlings¹

ABSTRACT

The effect of various eradicants and fertilizers used in nursery practice on the oxygen uptake of excised root tips of seedlings of jack pine, red pine, white pine, and black locust seedlings was investigated using manometric techniques. The compounds studied included calomel, spergon, thiosan, chlordane, Stoddard oil, and common fertilizer salts applied at ordinary rates. In the first phase of the study the root tips were placed directly into solutions or suspensions of various compounds and their rate of oxygen uptake determined. The second phase was concerned with the respiration of root tips of seedlings grown in cultures treated with the chemicals. The measurements show that some of the compounds applied to nursery soils are inhibitory to the metabolism of roots of young seedlings. The results also indicate that manometric methods of analysis provide a useful tool for the evaluation of the effect of various chemical compounds used in current nursery practice.

¹ Contribution from the Soils Department in cooperation with the Departments of Economic Entomology and Plant Pathology, Wisconsin Agricultural Experiment Station, Madison, Wis., and the Wisconsin State Conservation Department. Publication approved by the Director of the Wisconsin Agricultural Experiment Station. Presented before Division V-A, Soil Science Society of America, Cincinnati, Ohio, November 20, 1952.

² Instructor in Soils, University of Wisconsin. The author acknowledges the helpful suggestions of Drs. S. A. Wilde, A. J. Riker, and R. D. Shenefelt and Mr. J. G. Berbee, University of Wisconsin.

Received for publication December 6, 1952.