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Published in Soil Sci Soc Am J 19:175-179 (1955)
© 1955 Soil Science Society of America
677 S. Segoe Rd., Madison, WI 53711 USA
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Description of Two Bacteriophages Active Against Lotus Rhizobia1

C. W. Bruch and O. N. Allen2

ABSTRACT

The isolation and proliferation of four races of phage against rhizobia from nodules of Lotus corniculatus are described. Growth characteristics of two races, P-2 and P-11, are given in detail. Neither of these two phages showed differences in plaque morphology. Higher titers of these races were not obtained in media containing increased increments of yeast water and calcium as compared with modified medium 79. Optimum pH level for phage proliferation and viability ranged between pH 6.5 and 7.5. Each phage maintained its titer for 60 days upon storage at 2° C., but was increasingly less stable at 10° C., 25° C., and 37° C. Optimum temperature for proliferation was 27 to 30° C.; inactivation was rapid at 45° C., 50° C., and 55° C. Alterations in infectiveness and effectiveness, as judged by host plant response, were not obtained when (a) phage-resistant strains and (b) suspensions of phage particles and susceptible rhizobia (ratio 3:1) were used as inocula in greenhouse tests.

NOTES

1 Contribution from the Department of Bacteriology, University of Wisconsin, Madison. Published with the approval of the director, Wisconsin Agr. Exp. Sta. This work was supported in part by a grant from the Wisconsin Alumni Research Foundation. Presented before Div. III, Soil Science Society of America, St. Paul, Minn., Nov. 9, 1954.

2 Research Assistant. Present address: Chemical Corps, Camp-Detrick, Frederick, Md., and Professor of Bacteriology, respectively.

Received for publication October 25, 1954.




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Copyright © 1955 by the Soil Science Society of America.