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Effect of Some Long-Time Rotations on the Amino Acid Composition of the Soil¹

ABSTRACT

The technique of ion exchange chromatography has been further refined for the isolation and quantitative determination of the ninhydrin-positive components of soil hydrolysates. The method was used to determine the effect of some long-time rotations on the distribution of amino acids in soils. The soils used for this purpose were taken from the Morrow Plots at the University of Illinois.

It was found that these plots differed in both the proportion of the soil nitrogen present as -amino acid nitrogen and in the relative distribution of amino acids. The most striking difference was in the basic amino acid content of these soils, where a high quantity of basic compounds was associated with the untreated rotations. An explanation is given to account for these observations.

¹ Contribution from the Department of Agronomy, Illinois Agr. Exp. Sta., Urbana, Ill. Published with the approval of the Director.

² Assistant Professor in Soil Biology. Appreciation is expressed to Mr. Uldis Blukis and Mr. John B. Duncan for performing many of the determinations.

Received for publication July 28, 1955.