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ABSTRACT
This paper describes a procedure for use of O18 in soil aeration tracer studies. Atmolysis, isotopic exchange, and respiratory fractionation produce only small errors when using O16O18 as a tracer for O2 diffusing in soil under steady state conditions. A special train for producing equal, continuous streams of O18-enriched and unenriched air was developed. Analyses of experimental samples of oxygen were performed with a mass spectrometer. A split canopy experiment is described in which the effects of different surface conditions on the soil oxygen supply may be compared; in this case, the presence and absence of corn seedlings were compared. Results are expressed in terms of the mixing ratio of air from the two halves of the canopy. Improvements and possibilities of the method are discussed.
1 Joint Publication: Journal Paper No. J-4477 of the Iowa Agr. and Home Econ. Exp. Sta., Ames, Project No. 998, Department of Agronomy; and contribution No. 1384 of the Institute for Atomic Research, Ames, Iowa. Presented before Div. I, Soil Science Society of America, Nov. 30, 1961, at St. Louis, Mo.
2 Formerly Graduate Assistant, now with the Dept. of Soils and Plant Nutrition, University of California, Riverside; and Professor of Soils and Physics, Iowa State University, Ames, respectively.
Received for publication November 19, 1962. Accepted for publication January 31, 1963.
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