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Humification of Carbon-14 Labeled Glucose in Soils of Sanborn Field¹

ABSTRACT

Duing the incubation of C¹⁴-labeled glucose in soil for 3 months, 25% of the radioactive carbon was incorporated in the soil organic matter while the remainder was evolved as CO₂. Fellowing incubation, the soil organic matter was extracted by 0.5N NaOH and fractionated. Humin, humic, and fulvic fractions were similarly labeled in a given soil. Generally, soil organic matter resulting from glucose was rather uniform in character for the several soils studied, whereas, that present prior to adding glucose may have differed among soils as a result of humification of diverse substrates. Selective combustion of aliphatic material in each fraction by heating at 170C for 25 days indicated that about 50% of the total carbon in humin and fulvic material and 66% of that in humic acid were aromatic. Aromatic and aliphatic components of the fulvic fraction were about equally labeled in all soils. Humic acid and humin showed less C¹⁴ incorporation into the aromatic than the aliphatic component.

¹ Contribution from the Department of Soils, Missouri Agr. Exp. Sta., Missouri Journal Series no. 3056. Approved by the Director.

² Post Doctoral Fellow and Associate Professor of Soils, respectively, Univ. of Mo., Columbia.

Received for publication June 6, 1966. Accepted for publication July 15, 1966.