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The Use of Gas-Liquid Chromatography for the Determination of Sugars Extracted from Soils by Sulfuric Acid¹

ABSTRACT

A quantitative procedure was developed for the determination of soil sugars by gas-liquid chromatography of the acetate derivatives of the alditols obtained by reduction of the sugars. Myoinositol or quebrachitol was used as an internal standard during the production of alditol acetates and their separation by gas-liquid chromatography.

Sugars were isolated from soil hydrolysates by neutralization of the acid and precipitation of the sulfate using strontium carbonate. The filtrates were evaporated to dryness and the sugars extracted using methanol.

The release of monosaccharides from soils using H₂SO₄ was studied and conditions of hydrolysis are recommended which depend on the nature of the soil and the purpose of the investigation.

¹ Contribution from the Department of Agronomy, Missouri Agr. Exp. Sta., Missouri Journal Series no. 5773. Approved by the Director.

² Post Doctoral Research Associate on leave from Waite Agricultural Research Institute, University of Adelaide, S. Australia; Graduate Research Assistant; and Associate Professor of Agronomy, respectively, University of Missouri, Columbia. Dr. Kirkman is presently with Botanical Institute, Agricultural College of Norway.

Received for publication August 27, 1969. Accepted for publication November 20, 1969.