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Nitrogen Fixation by Bacteria in the Root Zone of Rice¹

ABSTRACT

Atmospheric nitrogen is fixed in the root zone of rice plants (Oryza sativa L.). Acetylene-ethylene assay showed that bacteria, rather than the root tissues, do the fixation. Although the root tissues produce a trace of ethylene, they cannot reduce acetylene to ethylene. The apparent K_m (Michaelis constant) of acetylene was much higher than values from known nitrogenfixing bacteria.

¹ Contribution from The International Rice Research Institute, Los Baños, Laguna, Philippines.

² Soil Microbiologist and Research Assistant, respectively. Mailing address: P. O. Box 583, Manila, Philippines.

Received for publication July 29, 1970. Accepted for publication October 22, 1970.