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A Modified Sand Culture Method for Rhizobium Assay¹

ABSTRACT

Usual methods of conducting Rhizobium-legume effectivity trials failed because of wild Rhizobium contamination caused by dust storms. A modified method was developed that successfully reduced contamination. The culture method was successful in the greenhouse and in the growth room.

¹ Contribution from the Soil and Water Conservation Research Division, Agricultural Research Service, USDA, in cooperation with the College of Agriculture, Washington State Univ., Pullman.

² Microbiologist and Soil Scientist, respectively, formerly located at Prosser, Washington, Present addresses: USDA-ARS-SWC, Univ. of Nebraska, Lincoln; and Battelle Northwest, Richland, Wash., respectively.

Received for publication October 9, 1970. Accepted for publication November 2, 1970.