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Interactions with Soil Lipase Activation and Inhibition¹

ABSTRACT

Soil lipase interactions of Psammetic paleustalf, Eufaula loamy sand, were determined with a fluorometric assay of enzymatic cleavage converting nonfluorescent 4-methyl umbelliferone butyrate (4-MUB) to the highly fluorescent 4-methyl umbelliferone (4-MU). Soil lipase activity accelerated with manipulation of base cation levels. Combination effectiveness as reaction activators at 2.0M x 10^-4 was Ca + Mg + Na or K or Na + K > Ca + Mg > Ca > Mg > Na or K. Highly significant competitive lipase inhibition was induced with 0.1M Cu²⁺ > S^2- > EDTA at MUB substrate levels of 12.5 to 75.0 x 10^-4M. Iron³⁺ noncompetitive inhibition was near constant.

¹ Journal article 2403 of the Agr. Exp. Sta., Oklahoma State Univ., Stillwater, Oklahoma 74074. This research was supported in part by PHS ES-00712 and American Cancer Society Research Grant T-562.

² Graduate Research Assistant and Professor, respectively.

Received for publication February 28, 1972. Accepted for publication September 7, 1972.