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Nitrogen-15-Depleted versus Nitrogen-15-Enriched Ammonium Sulfate as Tracers in Nitrogen Uptake Studies¹

ABSTRACT

Fertilizer particles were prepared from ammonium sulfate with a lower (0.031) than natural (0.366) atom percentage of ¹⁵N. The ¹⁵N-depleted material was compared with ammonium sulfate of a comparable level of ¹⁵N enrichment (0.734 atom %) in a greenhouse pot experiment which measured plant recovery of applied N. There was no statistical difference in N recovery values for five harvests taken over an 18-week period at three rates of applied tracer N, with on exception. Accurate and precise measurement of tracer N in plant tissue was obtained for both ¹⁵N-depleted and ¹⁵N-enriched materials, using two mass spectrometers with different measurement and rcording systems. A nontracer technique gave lower first harvest and higher combined harvest values of percentage recovery of applied N than either tracer technique.

¹ Contribution from the Soils and Fertilizer Research Branch, National Fertilizer Development Center, TVA Muscle Shoals, Ala. 35660.

² Research Soil Chemists.

Received for publication March 24, 1974. Accepted for publication June 23, 1974.