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Use of Selective Agents for Recovery of Rhizobium meliloti from Soil¹

ABSTRACT

This study was planned to develop a useful selective medium for differentiating Rhizobium meliloti in soils or inocula. The objective was to define a medium which will permit growth of rhizobia and restrain growth of other microorganisms which might inhibit or mask growth of the desired strains of Rhizobium. A yeast mannitol agar medium reinforced with 500 µg/ml pentachloronitrobenzene, 1.5 µg/ml brilliant green, 1.0 µg/ml sodium azide, and 25 µg/ml Congo red proved to be very effective in differentiating R. meliloti from other species of rhizobia as well as other forms of soil microorganisms native to Oregon. Antibiotic resistance as a marker can also be used in association with the selective medium to study the ecology of rhizobia in soil or other environments. However, antibiotic resistance is apparently not a stable character and deserves frequent testing, particularly in long-term studies.

¹ Contribution from the Department of Microbiology, Oregon State University, Corvallis, OR 97331.

² Formerly Assistant Professor. Now Assistant Professor, USDA-SEA, AR, Dept. of Microbiology, North Carolina State Univ., Raleigh, NC 27650.

Received for publication October 16, 1978. Accepted for publication July 19, 1979.