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An Agar Plate Method for Rapid Screening of Rhizobium for Tolerance to Acid-Aluminum Stress¹

ABSTRACT

An agar plate method has been developed for the rapid, preliminary screening of large numbers of rhizohia for tolerance to acidity and aluminum (acid-Al). Media containing pH indicators are used, and distinguish true tolerance from cases where growth depends on a previous pH shift. Strains which were highly tolerant or sensitive to acid Al on agar behaved similarly in defined liquid media. Strains that formed dry, pinpoint colonies were more sensitive to acid-Al than those that formed large, gummy colonies.

¹ Contribution from the International Institute of Tropical Agriculture (IITA), P.M.B. 5320, Ibadan, Nigeria, and the Dep. of Land, Air and Water Resources, Univ. of California, Davis, CA 95616. IITA Jour. Paper Ser. no. 217. Supported in part by a grant from the United Nations Development Programme (UNDP).

² Soil Microbiologists, IITA, Ibadan, Nigeria, and Professor of Soil Science, Univ. of California, Davis.

Received for publication June 7, 1982. Accepted for publication September 21, 1982.