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A New Plant Growth Technique for the Most-Probable-Number Estimation of Rhizobium¹

ABSTRACT

A new system for growing plants in the most-probable-number (MPN) estimation of Rhizobium is described. Beans, peanuts, and alfalfa were grown in small plastic cone-tainers and inoculated with suspensions of rhizobia. The number of rhizobia in pure culture, inoculants, and soil was satisfactorily estimated using cone-tainers for host plant growth. Most-probable-number counts of R. phaseoli were comparable in growth pouches and cone-tainers. The cone-tainer system saves bench space and costs less than other presently used techniques.

¹ Journal Article no. 969. New Mexico Agric. Exp. Stn., New Mexico State University, Las Cruces, NM 88003.

² Graduate Assistant and Assistant Professor, respectively, Crop and Soil Sciences Dep., New Mexico State Univ., Las Cruces, NM 88003.

Received for publication September 29, 1982. Accepted for publication January 4, 1983.