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Identification of Two Dominant Serotypes of Rhizobium trifolii in Root Nodules of Uninoculated Field-Grown Subclover¹

ABSTRACT

Analysis of the protein profile patterns by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of 30 isolates of Rhizobium trifolii obtained from root-nodules of uninoculated field grown plants of subclover (Trifolium subterraneum L.) cv. ‘Mt. Barker’ showed that 12 and 9 isolates were represented by two different protein profile patterns (gel types). Serological analyses performed using antisera raised to one isolate of each gel type showed that although the two isolates, 1-01 and 2-01, shared a common antigenic determinant, they were distinguishable by their reactions in whole-cell somatic agglutination and gel-immune-diffusion tests. Forty-nine of the sixty isolates collected from nodules in the establishment year, and 56 of 90 isolates collected the following year were antigenically identical to either one or the other of the two parent isolates. Sixteen of the one hundred fifty isolates cross-reacted strongly with both antisera in whole-cell somatic tube agglutination and gel-immune-diffusion assays while only 23 of the 150 isolates were antigenically unrelated to either 1-01 or 2-01. All of the 22 isolates of serotype 1-01 collected in the establishment year possessed the same gel type, wheres 19 of 27 isolates of serotype 2-01 had the same gel type as the parent isolate, 2-01. The majority (18 of 22) of isolates of serotype 1-01 were superior symbiotic effectiveness while the majority (17 of 19) of isolates of serotype 2-01 possessing the same gel type were of inferior effectiveness.

¹ Oregon State Univ. Agric. Exp. Stn. Tech. Paper no. 6973.

² Graduate Research Assistant, Dep. of Microbiology, and Associate Professor, Dep. of Microbiology and Soil Science, Oregon State Univ., Corvallis, OR 97331-3804, respectively.

Received for publication October 7, 1983. Accepted for publication March 27, 1984.