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Published in Soil Sci Soc Am J 49:448-452 (1985)
© 1985 Soil Science Society of America
677 S. Segoe Rd., Madison, WI 53711 USA
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Immobilization of Nitrogen-15-Labelled Urea in a Jack Pine Forest Floor1

N. W. Foster, E. G. Beauchamp and C. T. Corke2

ABSTRACT

Urea labelled with 15N was applied at 200 kg N ha–1 in the spring to a 45-yr-old natural jack pine (Pinus banksiana Lamb.) forest near Chapleau, ON. Fertilizer recovery in the L and F horizons was determined 32, 64 and 96 d after fertilization. Three months after fertilization, 25% of the added N had been immobilized in the forest floor and was recovered as organic N. The possibility that chemical reactions contribute to fertilizer N immobilization was investigated by applying urea to sterilized, mixed L and F horizon materials in an 8-d laboratory incubation study. The amount of N fixed chemically in urea-treated forest floor materials exceeded the amount of N immobilized in microbial tissue. The nature of the chemical reaction was investigated in a second incubation study. Urea did not react with L + F horizon materials either at the natural soil pH (4.5) or at the higher pH (8.0) resulting from the addition of KOH. Since urea was applied at a rate sufficient to raise soil pH to the alkaline range, immobilization may be explained by reaction between NH3 and soil organic matter.


NOTES

1 Contribution from the Government of Canada, Canadian Forestry Service, Great Lakes Forest Research Centre, Sault Ste. Marie, ON. From a thesis submitted by the senior author in partial fulfillment of the requirements for the Ph.D. degree at the Univ. of Guelph. Presented before Div. S-7, at the American Society of Agronomy meeting, Anaheim, CA, on 1 December 1982.

2 Research Scientist (Great Lakes Forest Research Centre), Professor, and Professor (retired), Univ. of Guelph, respectively.

Received for publication February 21, 1982. Accepted for publication October 10, 1984.




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