HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Nieto, K. F. Articles by Frankenberger, W. T. Search for Related Content PubMed Articles by Nieto, K. F. Articles by Frankenberger, W. T., Jr. Agricola Articles by Nieto, K. F. Articles by Frankenberger, W. T.

Single Column Ion Chromatography: I. Analysis of Inorganic Anions in Soils¹

ABSTRACT

A useful analytical method is described for the determination of inorganic mono-and divalent anions in soils. Single column ion chromatography (SCIC) is a rapid, sensitive, and precise method which can simultaneously detect Cl^–, NO^–₂, NO^–₃, SO^2–₃, and SO^2–₄ in soils in less than 10 min. In addition, Br^–, I^–, and ClO^–₄ can be separated within 15 min. The method can be carried out on a commercially available HPLC instrument, using a 500 µL aliquot of soil extract and an eluent stream of 4 mM phthalic acid (pH 4.5). The inorganic anions are separated on a low-capacity anion exchange column and quantified by conductimetric detection. Results obtained by the SCIC method agree closely with those obtained by other analytical methods: Cl^–, AgNO₃ titrimetric method (r = 0.987***); NO^–₂-N, diazonium coupling reaction (r = 0.993***); NO^–₃-N, steam distillation (r = 0.998***); SO^2–₄-S, autoanalyzer (r = 0.998***). Using a 500 µL loop, SCIC detection limits, expressed in mg/L of soil extract, were: Cl^–, Br^–, NO^–₃-N, and SO^2–₄-S (0.025), ClO^–₄ and I^– (0.5); NO^–₂-N and SO^2–₃-S (1.0). Several extracting reagents (water, LiCl, KCl, Ca[C₂H₃O₂l₂, and CaCl₂) were compared to determine the best soil extraction procedure. This method proved to be reproducible as evident by the low CV values ranging from 3.6 to 8.4%. Notable advantages of this technique include: (i) single column simplicity, (ii) application to a wide variety of inorganic anions, (iii) simultaneous determination of ions in soil extracts without interferences by soil constituents, and (iv) inherent sensitivity greater than most methods employed for the determination of inorganic soil anions.

¹ Contribution of the Dep. of Soil and Environ. Sci., Univ. of Calif., Riverside, CA 92521. Presented before Div. S-2, Soil Sci. Soc. of Am., Anaheim, CA 1 Dec. 1982.

² Graduate Student and Assistant Professor of Soil Science, Univ. of Calif., Riverside.

Received for publication February 10, 1984. Accepted for publication November 21, 1984.

This article has been cited by other articles:

				K. M. DeAngelis, P. Ji, M. K. Firestone, and S. E. Lindow Two Novel Bacterial Biosensors for Detection of Nitrate Availability in the Rhizosphere Appl. Envir. Microbiol., December 1, 2005; 71(12): 8537 - 8547. [Abstract] [Full Text] [PDF]