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Determination of FeEDDHA in Soils and Fertilizers by Anion Exchange Chromatography¹

ABSTRACT

The specific conditions for the quantitative analysis of FeEDDHA (ethylenedimine di-o-hydroxyphenylacetic acid) by high pressure liquid chromatography (HPLC) were studied and defined. Using a 3-cm anion exchange column and 5 mM H₂SO₄ + 0.01 mM Fe₂(SO₄)₃ as an eluent, FeEDDHA was separated into the dl-racemic and meso isomers. With the same column and 3 mM H₂SO₄ + 50 mM Na₂SO₄ + 0.01 mM Fe₂(SO₄)₃, FeEDDHA eluted as a single peak. Standard calibration curves for FeEDDHA were obtained with regression coefficients of 0.9999 at concentrations up to 0.25 mM. The coefficient of variation is 1.5%, or 1.8 µmol L^–1 using a 50-µL sample; the detection limit was 1.2 µmol L^–1. FeEDDHA was separated easily on anion exchange HPLC columns from other chelates such as CDTA, EDTA, and DTPA. The proposed method was used to determine FeEDDHA concentrations in water extracts of soils that were incubated with the compound. With variation of the eluent, separation of ferrated and unferrated EDDHA is possible.

¹ Contribution of the Seagram Center for Soil and Water Sciences, Faculty of Agriculture, Hebrew Univ. of Jerusalem, P.O. Box 12, Rehovot, Israel 76-100. This research was supported by the U.S.-Israel (Binational) Agricultural Research and Development Fund (BARD).

² Graduate Research Student and Professor, respectively, Seagram Center for Soil and Water Sciences, Faculty of Agriculture, Hebrew Univ. of Jerusalem, P.O. Box 12, Rehovot, Israel 76-100.

Received for publication July 29, 1986.