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Partitioning Dissolved Inorganic and Organic Phosphorus Using Acidified Molybdate and Isobutanol

Dep. of Plant Pathology

A. P. Schwab^*

Dep. of Agronomy, Throckmorton Hall, Kansas State Univ., Manhattan, KS 66506-5502

* Corresponding author.

ABSTRACT

Quantification of P-mineralization rate in soil using isotopic dilution requires that soluble inorganic ³²P (³²P_i) be determined without interference from soluble organic ³²P (³²P_o). Therefore, a method is needed to partition ³²P_i and ³²P_o into physically separate solutions before radiation counting. A previously suggested method of extracting P_i into isobutanol with acidified molybdate was tested for efficiency of separation from P_o. Inorganic P as KH₂PO₄ was completely recovered in the isobutanol phase with acid molybdate. Organic P (glycerophosphate) remained in the aqueous phase during the extraction. No P_o hydrolysis was observed when sodium phytate, glycerophosphate, ribonucleic acid, adenosine 2'- and 3'-monophosphate, and cytidine 2'- and 3'-monophosphate were extracted, but detectable amounts of adenosine 5'-triphosphate hydrolyzed. This technique was tested on four Kansas soils, and the levels of inorganic and organic P obtained by this method were the same as those detected by conventional analyses.

Contribution no. 90-509-J from the Kansas Agric. Exp. Stn.

Received for publication April 24, 1991.

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