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Published in Soil Sci Soc Am J 57:111-115 (1993)
© 1993 Soil Science Society of America
677 S. Segoe Rd., Madison, WI 53711 USA
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Fate of Nitrogen-15 Enriched Ammonium Nitrate Applied to Corn

G. B. Reddy*

Dep. of Plant Science and Technology, North Carolina A&T State Univ., Greensboro, NC 27411

K. R. Reddy

Dep. of Soil and Water Science, Univ. of Florida-IFAS, Gainesville, FL 32611

*Corresponding author.

ABSTRACT

Nitrogen utilization by corn (Zea mays L.) is influenced by the form of inorganic N present in the root zone. A field experiment was conducted on Enon sandy loam (fine, mixed, thermic Ultic Hapludalf) to determine N use efficiency and its partitioning in various plant parts of corn. Ammonium nitrate labeled either as 15NH4-N or 15NO3-N and applied at 50, 100, or 200 kg N ha–1 was evaluated. Microplots (1.06 m2) were established in the main N plots for 15N fertilizer application, and main plots were used to determine grain yield. After the crop harvest, soil N alone, fertilizer N used by the crop, and fertilizer N remaining in inorganic and organic forms in the top 75 cm of the soil were measured. Grain yield response to N application was significant (P < 0.01). Recovery of applied fertilizer N in corn and weeds ranged from 43 to 57% and 3 to 5%, respectively. Nitrogen sources showed no significant differences with respect to N accumulation in corn. Only 17 to 20% of the fertilizer N was recovered in the grain. The amounts of soil N utilized by the corn was about three-to sixfold higher than the fertilizer N, suggesting extensive turnover of soil and fertilizer N through immobilization and mineralization. More N was recovered (21–63% of added N) in the soil from 15NH4 than from 15NO3 (6–38%). Loss of N (unaccounted for) ranged from 11 to 18% at 100 kg N ha–1 and from 34 to 48% at 200 kg N ha–1. Nitrogen loss was higher in the plots receiving 15NO3 than 15NH4. Most of the fertilizer N remaining in the soil at the end of the growing season was in the organic fraction, suggesting immobilization into microbial and root biomass.


NOTES

Joint contribution from North Carolina A&T State Univ. and Univ. of Florida.

Received for publication February 21, 1992.


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