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Organic Matter Comparison of Wettable and Nonwettable Soils from Bentgrass Sand Greens

Ecogen, Inc., 2005 Cabot Blvd. West, Langhorne, PA 19047

S. J. Traina

Dep. of Agronomy, Ohio State Univ., Columbus, OH 43210

W. W. Shane

S.W. Michigan Research and Education Center, Benton Harbor, MI 49022

*Corresponding author.

ABSTRACT

Previous studies have attributed hydrophobicity in sandy soils to alkaline extractable macromolecular complexes, as well as lipid fractions, but have not included wettable soils for comparison. Our study was conducted to compare the alkaline-extractable and lipid fractions of a nonwettable soil to a wettable soil from creeping bentgrass (Agrostis stolonifera L. var. stolonifera) sand greens. Samples were collected from two sites and several extraction sequences performed on each. Humates were extracted in 1.25 mol L^–1 NaOH. Alkaline-extractable fractions were separated by precipitation with 6 mol L^–1 HCl. Acid precipitated and nonprecipitated fractions were purified, lyophilized, and analyzed by Fourier transform infrared spectroscopy. In addition, the acid nonprecipitated fractions, which included fulvic acids, were analyzed by solid-phase ¹³C nuclear magnetic resonance spectroscopy. Lipid fractions were extracted with a soxhlet apparatus using either hexane or methanol as solvent. Lipid fractions were analyzed by gas chromatography-mass spectroscopy. No qualitative differences were observed with Site 1 extracts, and it appeared that hydrophobicity may have been at least partly due to physical or structural differences. Qualitative differences were observed with Site 2 extracts when initially extracted with methanol.

Received for publication February 27, 1992.

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