HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Cattelan, A. J. Articles by Fuhrmann, J. J. Search for Related Content PubMed Articles by Cattelan, A. J. Articles by Fuhrmann, J. J. Agricola Articles by Cattelan, A. J. Articles by Fuhrmann, J. J.

Bacterial Composition in the Rhizosphere of Nodulating and Non-Nodulating Soybean

Dep. of Crop and Soil Sciences, 3111 Plant Sciences Bldg., Univ. of Georgia, Athens, GA 30602-7272

J. J. Fuhrmann

Dep. of Plant and Soil Sciences, Univ. of Delaware, Newark, DE 19717-1303

*Corresponding author (pghartel{at}arches.uga.edu).

ABSTRACT

Little is known about the particular species of bacteria that are favored for growth in the rhizosphere. Obtaining this information will facilitate a better understanding of root colonization and competitiveness of rhizosphere organisms. We used fatty acid methyl ester (FAME) analysis to identify bacteria in two different soils and in the rhizosphere of nodulating and non-nodulating ‘Lee’ soybean [Glycine max (L.) Merr.]. In a light-room experiment, bacterial isolates from each soil and soybean rhizosphere were selected at 3 (emergence), 6 (V1 stage) and 15 (V2–V3 stage) d after planting (DAP). Of 1131 bacterial isolates examined, 572 (50.6%) were identified. The majority of the identified isolates were Bacillus (324 isolates), Pseudomonas (121 isolates), and Arthrobacter (48 isolates). Of the 49 total bacterial species identified, 29 were detected in only one soil or the other. Numbers of Bacillus spp. were lower in the rhizosphere of nodulating and non-nodulating soybean than in root-free soil, and these lower numbers were usually offset by higher numbers of Gram-negative bacteria, especially Pseudomonas spp. Principal components analysis showed a distinct clustering in each of the two soils. The percentages of isolates identified from each habitat, and the clusters derived from the FAME analysis, showed differences between soybean rhizosphere and root-free soil and, to a lesser extent, between nodulating and non-nodulating soybean rhizosphere. These results suggest that each soil offered specific populations of bacteria and from these populations, soybean roots favored growth of certain bacteria. To a lesser extent, this specificity was also apparent between nodulating and non-nodulating soybean.

Received for publication January 3, 1997.

This article has been cited by other articles:

				C. E. Morris, M. Bardin, O. Berge, P. Frey-Klett, N. Fromin, H. Girardin, M.-H. Guinebretiere, P. Lebaron, J. M. Thiery, and M. Troussellier Microbial Biodiversity: Approaches to Experimental Design and Hypothesis Testing in Primary Scientific Literature from 1975 to 1999 Microbiol. Mol. Biol. Rev., December 1, 2002; 66(4): 592 - 616. [Abstract] [Full Text] [PDF]

				L. L. Daane, I. Harjono, G. J. Zylstra, and M. M. Häggblom Isolation and Characterization of Polycyclic Aromatic Hydrocarbon-Degrading Bacteria Associated with the Rhizosphere of Salt Marsh Plants Appl. Envir. Microbiol., June 1, 2001; 67(6): 2683 - 2691. [Abstract] [Full Text]

				C.-H. Yang, D. E. Crowley, J. Borneman, and N. T. Keen Microbial phyllosphere populations are more complex than previously realized PNAS, March 27, 2001; 98(7): 3889 - 3894. [Abstract] [Full Text] [PDF]

				A.J. Cattelan, P.G. Hartel, and J.J. Fuhrmann Screening for Plant Growth-Promoting Rhizobacteria to Promote Early Soybean Growth Soil Sci. Soc. Am. J., November 1, 1999; 63(6): 1670 - 1680. [Abstract] [Full Text] [PDF]