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SOIL & WATER MANAGEMENT & CONSERVATION

Sample Pretreatment and Phosphorus Speciation in Wetland Soils

^a Smithsonian Tropical Research Institute, Apartado 0843-03092, Balboa, Ancón, Republic of Panama
^b Everglades Division, South Florida Water Management District, 3301 Gun Club Rd., West Palm Beach, FL 33406
^c Wetland Biogeochemistry Lab., Soil and Water Science Dep., Univ. of Florida, 106 Newell Hall, P.O. Box 110510, Gainesville, FL 32611

^* Corresponding author (turnerbl{at}si.edu).

We assessed the influence of sample pretreatment on the amounts and forms of P extracted in NaOH–EDTA (ethylenediamine tetraacetic acid) from a series of contrasting wetland soils from the Florida Everglades. Samples of unconsolidated benthic floc and underlying soil (0–10 cm) were extracted either fresh (overnight refrigeration only), air dried (10 d at 30°C), or frozen at –80°C and lyophilized (48 h), before extraction and solution ³¹P nuclear magnetic resonance (NMR) spectroscopy. Significant differences in total P extraction following pretreatment were detected for one out of four benthic floc samples and three out of four soil samples, although the changes were inconsistent: in two cases the total P extraction increased, while in two others it decreased. Assessment of the P composition by solution ³¹P NMR spectroscopy revealed differences among treatments, although these were mostly within the range of error associated with replicate analyses; however, DNA was not detected in a fresh sample of calcareous benthic floc, despite representing an important component of the organic P extracted from dried samples. The apparent sample-specific nature of the changes confirms the importance of carefully assessing pretreatment effects in studies of soil organic P in wetlands.

Abbreviations: EDTA, ethylenediamine tetraacetic acid • NMR, nuclear magnetic resonance