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Organic Phosphorus in Soil Size Separates Characterized by Phosphorus-31 Nuclear Magnetic Resonance and Resin Extraction

^a Dep. of Crop Physiology and Soil Science, Danish Inst. of Agricultural Sciences, Research Centre Foulum, P.O. Box 50, DK-8830 Tjele, Denmark
^b Univ. of Bayreuth, Inst. of Soil Science and Soil Geography, 95440 Bayreuth, Germany

View larger version (20K): [in a new window]   Fig. 1 Solution 31P-nuclear magnetic resonance spectra of dialyzed NaOH extracts obtained from the sand, silt, and clay separates of the Taubenberg soil under spruce forest (Sf), deciduous forest (Df), permanent grassland (Gp), and arable rotation (Ar)

View larger version (16K): [in a new window]   Fig. 2 Solution 31P-nuclear magnetic resonance spectra of dialyzed NaOH extracts obtained from the sand silt and clay separates of the Askov soil under unfertilized (0), inorganic fertilized (2NPK), and animal manure (2AM) treatments

View larger version (31K): [in a new window]   Fig. 3 Percentage of the whole 31P-nuclear magnetic resonance spectral area allocated to each particular P form in the size separates of the Taubenberg soils. Apart from these quantified P forms in the figure, traces of inorganic orthophosphate were detected in all clay separates, traces of phosphonates were detected in spruce forest (Sf) and deciduous forest (Df) clay, and a trace of pyrophosphate was found in permanent grassland (Gp) clay. Ar is arable rotation

View larger version (27K): [in a new window]   Fig. 4 Percentage of the whole 31P-nuclear magnetic resonance spectral area allocated to each particular P form in the size separates of the Askov soils. Apart from these quantified P forms, traces of phosphonates were found in the unfertilized (0) and inorganic fertilized (NPK) treatment clays, and traces of inorganic orthophosphate were found in NPK and animal manure (AM) treatment clays

View larger version (16K): [in a new window]   Fig. 5 Functional relations between concentrations of Po extracted by resin (X) and the percentage of spectral area allocated to diester-P including teichoic acid-P (Y) for whole soils and for clay and silt separates. The full lines show the functional relations with the gradient b and the dotted lines are obtained by linear regression of Y on X (gradient = bYX) and X on Y (gradient = 1/bXY), respectively. For whole soils, the gradient b was 0.133, bYX was 0.092, and 1/bXY was 0.391; the correlation coefficient r2 was 0.235. For the size separates, the gradient b was 0.974, bYX was 0.862 and 1/bXY was 0.928 and the correlation coefficient r2 was 0.929. Land-use abbreviations are: Ar, arable rotation; Df, deciduous forest; Gp, permanent grassland; and Sf, spruce forest. Fertilizer-treatment abbreviations are: 0, unfertilized treatment; 2AM, animal manure treatment; 2NPK, inorganic fertilized treatment