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Procedure for Determining the Biodegradation of Radiolabeled Substrates in a Calcareous Soil

School of Agricultural and Forest Sci., Univ. Wales-Bangor, Gwynedd, LL57 2UW, UK

View larger version (42K): [in a new window]   Fig. 1. Schematic representation of the 14CO2 recovery system. Step 1 involves the addition of radiolabeled compounds to soil, incubation, and trapping any 14CO2 evolved with a static NaOH trap. Step 2 involves the release of any additional 14CO2 held in the soil by application of HCl and trapping evolved 14CO2 with a series of forced-air NaOH traps. The figure is drawn to scale

View larger version (32K): [in a new window]   Fig. 2. Microbial degradation of organic acids as measured by microbially respired 14CO2 in a high pH calcareous Typic Rendoll and low pH Typic Fragiochrept soil. (A) Low (0.005 µmol g-1) and (B) high (5 µmol g-1) organic acid addition to the soil. Clear bars represent the amount of 14CO2 recovered in a static NaOH-trap (Step 1), while striped bars represent the amount of 14CO2 recovered after the addition of HCl to the soil (Step 2; see Materials and Methods for details). Values represent means ± standard error of the mean, n = 3 (n.d. indicates not detectable)

View larger version (23K): [in a new window]   Fig. 3. Recovery (percentage of added NaH14CO3) of 14CO2 following the addition and incubation of a 14C-labeled bicarbonate (NaH14CO3) solution (0.001, 0.01, and 0.1 µmol g-1 soil) in a high pH calcareous Typic Rendoll (T. Rend.) or low pH Typic Fragiochrept (T. Frag.) soil. Hydrogen chloride (1 M) was added to the soil after 6 h (denoted by arrow). Values represent means ± standard error of the mean, n = 3