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Evaluation of the Microwave Irradiation Method for Measuring Soil Microbial Biomass

Dep. of Natural Resources, 80 Meiers Rd, Indooroopilly, Brisbane, Qld 4068, Australia

View larger version (18K): [in a new window]   Fig. 1. Temperature variation of water samples after microwave irradiation at 266 J mL-1, as affected by sample location and distribution in the oven.

View larger version (28K): [in a new window]   Fig. 2. Effect of soil weight on soil temperature after microwave irradiation at 248 J g-1. LSD0.01 = 9.0.

View larger version (49K): [in a new window]   Fig. 3. Effect of soil texture on soil temperature after microwave irradiation separately (one soil type) or together (all three soil types).

View larger version (19K): [in a new window]   Fig. 4. Increases in soil temperature, extractable C, and optical density of soil extracts after different periods of microwave irradiation.

View larger version (28K): [in a new window]   Fig. 5. Relationships between microbial biomass C (mg kg-1) measured by different methods. MIE: microwave irradiation-extraction method; SIR: substrate-induced respiration method; CFE: chloroform-fumigation extraction method; and CFI: chloroform-fumigation incubation method. r0.05 = 0.36, r0.01 = 0.46 when n = 30; r0.05 = 0.42, r0.01 = 0.54 when n = 22. The open circles represent soils with pH > 7.

View larger version (19K): [in a new window]   Fig. 6. Optical densities of 0.5 M K2SO4 extracts of soils following microwave irradiation or chloroform fumigation. The vertical bars on top of each column refer to standard errors of two replicates. LSD0.05 = 0.0068.