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Binding of DNA from Bacillus subtilis on Montmorillonite–Humic Acids–Aluminum or Iron Hydroxypolymers

Effects on Transformation and Protection against DNase

^a Dipartimento di Biologia e Chimica Agroforestale ed Ambietale, Univ. di Bari, Via Amendola 165/A, 70126 Bari, Italy
^b Dipartimento di Scienze Animali, Vegetali, e dell'Ambiente, Univ. del Molise, Via De Sancits s/n, 86100 Campobasso, Italy
^c Lab. of Microbial Ecology, Dep. of Biology, New York Univ., 100 Washington Sq. East, New York, NY 10003, USA

View larger version (16K): [in a new window]   Fig. 1. (a) Equilibrium adsorption of DNA on Al–M–HA. (b) Equilibrium adsorption of DNA on Fe–M–Ha. Data expressed as the means ± the SEs of the means (SEMs). Where not reported, SEMs are within the dimensions of the symbols.

View larger version (16K): [in a new window]   Fig. 2. (a) Amounts of DNA adsorbed on Al– or Fe–M–HA as a function of the cation-exchange capacity (CEC) of the complexes. Data were obtained by interpolating the best-fitting Langmuir, Freundlich, or linear equations, as reported in Table 3. (b) Percentage of DNA adsorbed on Al– or Fe–M–HA as a function of the organic C content of the complexes.

View larger version (16K): [in a new window]   Fig. 3. Effect of pH on the percentage of adsorption of DNA on Al– or Fe–M–HA complexes. Data expressed as the means ± the standard errors of the means (SEMs). Where not reported, SEMs are within the dimensions of the symbols.

View larger version (39K): [in a new window]   Fig. 4. Transformation of Bacillus subtilis by DNA free or bound on Al– or Fe–M–HA and effects of DNase, free or bound on Al–M–HA or Fe–M–HA, on transformation. Data expressed as the means ± SEs of the means.